H7N9

50%off> Cytokines and Growth Factors 10000 reagents

Antibody Purification Resins Protein A,G,L

Mouse mAb isotyping kit. No miss-match!!

All Antibodies (3000+)
  • Primary antibodies
  • Secondary antibodies
  • Tag antibodies
  • Myc Tag Antibody
  • Isotype control antibodies
  • Loading control antibodies
  • Beta-Actin Antibody
  • Tubulin Antibody
  • Histone H3 Antibody
Epidermal Growth Factor Receptor (EGF)
  • Epidermal Growth Factor (EGF) Receptors
  • Human Epidermal Growth Factor
Custom Services
  • Antibody Production Service
  • Antibody Purification Service
  • Antibody Development Service
  • Protein Production Service

IHC Support & Products

Coronavirus

  • Novel Coronavirus 2012 (NCoV)
  • Coronavirus Symptoms / NCoV Symptoms
  • New SARS-like Virus
  • SARS Coronavirus
  • Human Coronavirus
  • Coronavirus Vaccine
  • Novel Coronavirus (NCoV) Infection
  • Coronavirus Treatment / NCoV Treatment
  • Coronavirus Vaccine
  • Human Coronavirus (HCoV) Protein & Antibody
  • Novel Coronavirus (HCoV-EMC/2012) Spike & Antibody
  • Novel coronavirus (HCoV-EMC/2012) Nucleoprotein & Antibody
  • Human Cronavirus (HCoV) Spike Glycoprotein & Antibody
  • Human Coronavirus (HCoV) HKU1 Spike & Antibody

H7N9

  • H7N9 Proteins & Antibodies
  • H7N9 HA / Hemagglutinin Protein
  • Influenza A H7N9 NA / Neuraminidase Proteins
  • H7N9 M1 / Matrix Protein 1
  • H7N9 Antibodies
  • H7N9 cDNA Clones
  • Influenza A/Anhui/1/2013 (H7N9)
  • Influenza A/Shanghai/1/2013 (H7N9)
  • Influenza A/Hangzhou/1/2013 (H7N9)
  • Influenza A/Pigeon/Shanghai/S1069/2013 (H7N9)
  • H7N9 HA / Hemagglutinin Proteins & Antibodies

Troubleshooting - No staining


Immunohistochemistry (IHC) troubleshooting for no staining, high background and non-specific staining.

 

Problems

Possible reasons

Solutions

No staining

Wrong primary antibody

Choose an antigen-specific antibody

Inappropriate concentration (usually too low) of primary or secondary antibody

Optimize the concentration

The activities of primary/secondary antibody lost.

Check the antibody storage condition and run a positive control

Inappropriate pH of antibody diluents

Make sure antibody works properly

Insufficient deparaffinization

Deparaffinize sections longer

Insufficient permeabilization

Add an appropriate permeabilizing agent

Insufficient antigen retrieval

Follow the antigen retrieval protocol

The secondary antibody was not stored in the dark

Biotinylated secondary antibody should be stored in the dark.

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