H7N9

50%off> Cytokines and Growth Factors 10000 reagents

Antibody Purification Resins Protein A,G,L

Mouse mAb isotyping kit. No miss-match!!

All Antibodies (3000+)
  • Primary antibodies
  • Secondary antibodies
  • Tag antibodies
  • Myc Tag Antibody
  • Isotype control antibodies
  • Loading control antibodies
  • Beta-Actin Antibody
  • Tubulin Antibody
  • Histone H3 Antibody
Epidermal Growth Factor Receptor (EGF)
  • Epidermal Growth Factor (EGF) Receptors
  • Human Epidermal Growth Factor
Custom Services
  • Antibody Production Service
  • Antibody Purification Service
  • Antibody Development Service
  • Protein Production Service

IHC Support & Products

Coronavirus

  • Novel Coronavirus 2012 (NCoV)
  • Coronavirus Symptoms / NCoV Symptoms
  • New SARS-like Virus
  • SARS Coronavirus
  • Human Coronavirus
  • Coronavirus Vaccine
  • Novel Coronavirus (NCoV) Infection
  • Coronavirus Treatment / NCoV Treatment
  • Coronavirus Vaccine
  • Human Coronavirus (HCoV) Protein & Antibody
  • Novel Coronavirus (HCoV-EMC/2012) Spike & Antibody
  • Novel coronavirus (HCoV-EMC/2012) Nucleoprotein & Antibody
  • Human Cronavirus (HCoV) Spike Glycoprotein & Antibody
  • Human Coronavirus (HCoV) HKU1 Spike & Antibody

H7N9

  • H7N9 Proteins & Antibodies
  • H7N9 HA / Hemagglutinin Protein
  • Influenza A H7N9 NA / Neuraminidase Proteins
  • H7N9 M1 / Matrix Protein 1
  • H7N9 Antibodies
  • H7N9 cDNA Clones
  • Influenza A/Anhui/1/2013 (H7N9)
  • Influenza A/Shanghai/1/2013 (H7N9)
  • Influenza A/Hangzhou/1/2013 (H7N9)
  • Influenza A/Pigeon/Shanghai/S1069/2013 (H7N9)
  • H7N9 HA / Hemagglutinin Proteins & Antibodies

Immunohistochemistry (IHC) Protocol-Paraffin Section Protocol


  1. Fix dissected tissues with 10% formalin for no less than 48 hours at room temperature. Inadequately fixation can make tissues dehydrated during tissue processing and become hard and brittle.
  2. Rinse the tissue with running tap water for 30min-40min to eliminate the formaldehyde
  3. Dehydrate the tissues in EtOH baths in the following order:                                                         70% Ethanol 20 min (x1); 95% Ethanol 20 min (x2); 100% Ethanol 20 min (x2)
  4. Clear the tissue in xylene for 2 times, 20 min each.
  5. Melt the paraffin prior to adding the tissue. Incubate the tissue in a 65 °C paraffin bath for 2 times, 30 min each.
  6. Pour melted paraffin into paraffin block mold. Place the tissue well in the mold and wait for its cooling down. (15-20 min)
  7. Section the paraffin-embedded tissue block in 4-10 μm thickness slides on a microtome and float in a 37°C water bath containing deionized water.
  8. Float the sections onto clean glass slides and microwave at 65°C for 15 min, then the tissue binds to the glass. Slides can be stored overnight at room temperature or be used in immunohistochemical staining immediately.
  • Advantages:

1. Paraffin section slides can be stored at room temperature for a long time.

2.Paraffin section is more suitble to reveal distribution of target antigen compared to frozen section.

  • Disadvantages:

Epitopes of target antigens are likely to be damaged by high temperature or fixative in the whole process and a antigen retrieval procedure is needed.